Home > Sandwich ELISA Kits > Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

Product Name:

Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit

Cat.#:

JL41766

Regulatory Status:

RUO

Alternative:

C-Myc; BHLHE39; Myc Proto-Oncogene Protein; VMyc Class E basic helix-loop-helix protein 39; Transcription factor p64; Proto-oncogene c-Myc

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

1.56-100ng/mL

Sensitivity:

0.76ng/mL

Specificity:

This kit recognizes Human MYC in samples. No significant cross-reactivity or interference between Human MYC and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| MYC

Myc is a family of regulator genes and proto-oncogenes that code for transcription factors. The Myc family consists of three related human genes: c-myc (MYC), l-myc (MYCL), and n-myc (MYCN). c-myc (also sometimes referred to as MYC) was the first gene to be discovered in this family, due to homology with the viral gene v-myc. In cancer, c-myc is often constitutively (persistently) expressed.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	100	50	25	12.5	6.25	3.12	1.56	0
OD	2.51	1.96	1.24	0.72	0.46	0.3	0.22	0.09
Corrected OD	2.42	1.87	1.15	0.63	0.37	0.21	0.13	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human MYC spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	92-105	102
Cell Lysates(n=8)	96-109	105

| Linearity

Samples were spiked with high concentrations of Human MYC and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	88-97	90-110
1 : 2	Average (%)	93	96
1 : 4	Range (%)	87-108	105-115
1 : 4	Average (%)	98	108